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necrosis quantitation kit  (Biotium)


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    Biotium necrosis quantitation kit
    Necrosis Quantitation Kit, supplied by Biotium, used in various techniques. Bioz Stars score: 93/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/necrosis quantitation kit/product/Biotium
    Average 93 stars, based on 49 article reviews
    necrosis quantitation kit - by Bioz Stars, 2026-03
    93/100 stars

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    Inhibition of protein biosynthesis and induction of <t>apoptosis</t> in prostate cancer cells after treatment with EGF-PE24mutΔREDLK and SO1861. (A) Protein biosynthesis inhibition was demonstrated by puromycin Western Blot after 48 h in LNCaP and after 72 h in DU145 and PC-3 cells. EGFR-negative CHO cells remained unaffected. (B) Induction of apoptosis after combination treatment was marked by poly (ADP-ribose) polymerase (PARP) cleavage and caspase-3 activation. Western blots after 48 or 72 h of single or combination treatment of LNCaP (48 h), DU145, PC-3 or CHO cells (all 72 h) with 1.0 µg/ml SO1861 and 2.5 nM EGF-PE24mutΔREDLK. β-actin was used as a loading control. * As CHO cells are not of human origin, detection of human Caspase-3 was not possible in this cell line. Abbreviation: M, protein marker.
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    Inhibition of protein biosynthesis and induction of apoptosis in prostate cancer cells after treatment with EGF-PE24mutΔREDLK and SO1861. (A) Protein biosynthesis inhibition was demonstrated by puromycin Western Blot after 48 h in LNCaP and after 72 h in DU145 and PC-3 cells. EGFR-negative CHO cells remained unaffected. (B) Induction of apoptosis after combination treatment was marked by poly (ADP-ribose) polymerase (PARP) cleavage and caspase-3 activation. Western blots after 48 or 72 h of single or combination treatment of LNCaP (48 h), DU145, PC-3 or CHO cells (all 72 h) with 1.0 µg/ml SO1861 and 2.5 nM EGF-PE24mutΔREDLK. β-actin was used as a loading control. * As CHO cells are not of human origin, detection of human Caspase-3 was not possible in this cell line. Abbreviation: M, protein marker.

    Journal: Journal of Cancer

    Article Title: Synergistic Cytotoxicity of a Toxin Targeting the Epidermal Growth Factor Receptor and the Glycosylated Triterpenoid SO1861 in Prostate Cancer

    doi: 10.7150/jca.85691

    Figure Lengend Snippet: Inhibition of protein biosynthesis and induction of apoptosis in prostate cancer cells after treatment with EGF-PE24mutΔREDLK and SO1861. (A) Protein biosynthesis inhibition was demonstrated by puromycin Western Blot after 48 h in LNCaP and after 72 h in DU145 and PC-3 cells. EGFR-negative CHO cells remained unaffected. (B) Induction of apoptosis after combination treatment was marked by poly (ADP-ribose) polymerase (PARP) cleavage and caspase-3 activation. Western blots after 48 or 72 h of single or combination treatment of LNCaP (48 h), DU145, PC-3 or CHO cells (all 72 h) with 1.0 µg/ml SO1861 and 2.5 nM EGF-PE24mutΔREDLK. β-actin was used as a loading control. * As CHO cells are not of human origin, detection of human Caspase-3 was not possible in this cell line. Abbreviation: M, protein marker.

    Article Snippet: Quantification of apoptosis and necrosis after treatment was analyzed using the Apoptosis and Necrosis Quantification Kit Plus in accordance with the manufacturer's protocol (Biotium, Fremont, CA, USA).

    Techniques: Inhibition, Western Blot, Activation Assay, Control, Marker

    Induction of apoptosis and necrosis in prostate cancer cells after treatment with EGF-PE24mutΔREDLK and SO1861. (A) Cell morphology in phase-contrast microscopy at 20× magnification after 48 h or 72 h single or combination treatment with 2.5 nM EGF-PE24mutΔREDLK plus 1.0 µg/ml SO1861. Morphological changes during apoptosis were cell rounding, shrinking, blebbing (dotted arrow) and formation of apoptotic bodies (arrows). (B) Cells were stained with AV (FL1-H) and EthD-III (FLH-2) and analyzed by flow cytometry to distinguish between living cells (AV - / EthD-III - ), early apoptosis (AV + / EthD-III - ), late apoptosis (AV + / EthD-III + ) and necrosis (AV - / EthD-III + ).

    Journal: Journal of Cancer

    Article Title: Synergistic Cytotoxicity of a Toxin Targeting the Epidermal Growth Factor Receptor and the Glycosylated Triterpenoid SO1861 in Prostate Cancer

    doi: 10.7150/jca.85691

    Figure Lengend Snippet: Induction of apoptosis and necrosis in prostate cancer cells after treatment with EGF-PE24mutΔREDLK and SO1861. (A) Cell morphology in phase-contrast microscopy at 20× magnification after 48 h or 72 h single or combination treatment with 2.5 nM EGF-PE24mutΔREDLK plus 1.0 µg/ml SO1861. Morphological changes during apoptosis were cell rounding, shrinking, blebbing (dotted arrow) and formation of apoptotic bodies (arrows). (B) Cells were stained with AV (FL1-H) and EthD-III (FLH-2) and analyzed by flow cytometry to distinguish between living cells (AV - / EthD-III - ), early apoptosis (AV + / EthD-III - ), late apoptosis (AV + / EthD-III + ) and necrosis (AV - / EthD-III + ).

    Article Snippet: Quantification of apoptosis and necrosis after treatment was analyzed using the Apoptosis and Necrosis Quantification Kit Plus in accordance with the manufacturer's protocol (Biotium, Fremont, CA, USA).

    Techniques: Microscopy, Staining, Flow Cytometry